Application of the original ELISA kit and possible drawbacks

Probe domestic double-head probe 038-BB-5.7L The shape of the two ends of the needle is outside the pointed needle

EL-C1600N100013-B

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The basic principle of the original elisa kit 1) The antigen or antibody can be linked to the enzyme by a covalent bond to form an enzyme conjugate, and the enzyme conjugate can still maintain its immunological and enzymatic activity; 2) the antigen or antibody can be physically Sexually adsorbed on the surface of the solid support, it may be that the hydrophobic part between the surface of the protein and the polystyrene adsorbs to each other and maintains its immunological activity; 3) after the enzyme conjugate is combined with the corresponding antigen or antibody, the substrate can be added according to The color reaction determines whether there is an immune response, and the depth of the color reaction is proportional to the amount of the corresponding antigen or antibody in the specimen. Therefore, the test result can be displayed according to the degree of color development of the substrate. The possible drawbacks of the original ELISA kit ELISA is currently the most widely used immunolabeling method. However, due to the defects of ELISA itself and some human factors, experimental errors (leakage) can occur, and the analysis causes experimental error factors. In addition to human factors, the shortcomings affecting the original ELISA kit are as follows: 1) Transport and storage of reagents ELISA kits have poor thermal stability, and sensitivity and specificity will decrease after exposure to heat. Placed at 37 Â° C for 1 d is equivalent to storage at 4 Â° C ~ 10 Â° C for 1.6 months, while the shelf life of the ELISA kit is only 6 months, so the kit is required to be transported at 4 Â° C ~ 10 Â° C, laboratory teachers The requirements can be met in the storage of the kit, but the cold chain transport of the kit is not guaranteed. In the hot summer, the kit will be consumed for a long time after a few days of high temperature and long-distance transportation. The laboratory also uses the expiration date of the reagent instructions for up to 6 months, and the accuracy of the test results cannot be guaranteed. 2) Kit quality The quality of the kit is the core issue related to the accuracy of the ELISA test. At present, there are many manufacturers and brands of ELISA kits, and the product quality is also uneven. The original ELISA kit is mainly used in the following aspects: 1) A small amount of immunoprecipitation is observed. 2) Immunoenzyme staining for localization of various intracellular components. 3) Quantitative detection of antigen or antibody components in body fluids. 4) Study the synthesis of anti-enzyme antibodies.

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