Approaches human interleukin-1β (IL-1β) elisa kit - Database & Sql Blog Articles

Kaixin micro test

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Test probe PH-5H

EL-C1600N100013-B

Introduction to the basic information of human interleukin-1β (IL-1β) elisa kit

Introduction to IL-1

Also known as lymphocyte stimulating factor.

Cell source

It is mainly produced by activated mononuclear-macrophages.

Existing form

IL-1α and IL-1β.

Main biological function

(1) Local low concentration - immune regulation: synergistic stimulation of APC and T cell activation, promote B cell proliferation and secretion of antibodies.

(2) Mass production - endocrine effect: induces acute protein synthesis in the liver; causes fever and cachexia.

The general nature of IL-1

1. IL-1 production cells IL-1 are mainly produced by macrophages; in addition, almost all nucleated cells, such as B cells, NK cells, T cells cultured in vitro, keratinocytes, dendritic cells, astrocytes, IL-1 can be produced by fibroblasts, neutrophils, endothelial cells, and smooth muscle cells. Under normal circumstances, only skin, sweat and urine contain a certain amount of IL-1, and most cells can synthesize and secrete IL-1 after being stimulated by foreign antigen or mitogen.

2. IL-1 molecule IL-1 has two different molecular forms, one is called IL-1α, which is composed of 159 amino acids; the other is called IL-1β, which contains 153 amino acids; both are encoded by different genes. . Although the amino acid sequence has only 26% homology, IL-1α and IL-1β bind to the same cell surface receptor with the same affinity and exert the same biological effects.

3. IL-1 receptor (IL-1R) IL-1R is present on almost all nucleated cell surfaces, and the number of IL-1R varies from tens of (such as T cells) to thousands ( Such as fibroblasts). There are two main types of IL-1R: one is IL-1R1, the peptide chain part of the molecule extending into the cytoplasm is longer and plays a role of transmitting an activation signal; the other is IL-1R2, the intracellular part. The peptide is short and cannot effectively transmit signals. Instead, the extracellular portion of the peptide chain is released into the extracellular fluid and binds to IL-1 in a free form to exert feedback inhibition. GM-CSF, G-CSF and IL-1 can increase the expression level of IL-1R in cells, while TGF and corticosteroids can decrease the expression of IL-1R.

Approaching human interleukin-1β (IL-1β) elisa kit operation method

1. Dilute the antibody to 1-10 ug/ml with buffer. 0.1 ml was added to the reaction well at 4 ° C overnight. The next day, the solution in the well was discarded and washed 3 times with washing buffer.

2. Loading: Add 0.05 ml of the sample to be tested to the above-mentioned coated reaction well, and incubate at 37 ° C for 1 hour. Then wash (both blank wells, negative control wells and positive control wells).

3. Add the enzyme-labeled antibody: Add freshly diluted enzyme-labeled antibody (diluted by titration) to 0.05 ml in each reaction well. Incubate at 37 ° C for 0.5 to 1 hour and wash.

4. Add substrate liquid to develop color: Add 0.1 ml of temporarily prepared TMB substrate solution to each reaction well at 37 ° C for 10 to 30 minutes.

5. Stop the reaction: add 2M sulfuric acid 0.05ml to each reaction well.

6. Judgment of results: The results can be observed directly on the white background with the naked eye: the darker the color in the reaction well, the stronger the positive degree, the negative reaction is colorless or very light, according to the depth of the color, with "+", The "-" sign indicates. The OD value can also be measured: on the ELISA detector, at 450 nm (if the color is developed by ABTS, 410 nm), the OD value of each well is measured after zero adjustment of the blank control well, if it is greater than 2.1 times the OD value of the specified negative control. Is positive.

The advantages of our elisa kit: simple, fast, high sensitivity, specificity, cost-effective, and low sample requirements

1. The operation is simple and fast. All the steps, there are maps, so that you can be simple and clear, no need to delay the time for research steps.

2. High sensitivity. The enzyme is an organic catalyst, and a small amount of enzyme can induce a large amount of catalytic reaction, resulting in an observation color reaction phenomenon. Our kits quantify them at ug, ng, and pg levels.

3. Strong specificity. Specificity is derived from the selectivity of the antibody or antigen. The use of internationally renowned imported antibody antigens guarantees the quality of the kit.

4. High cost performance and stable quality.

5. The amount of sample required is small.

If you need to know the details of this product, please feel free to contact us.