Points to pay attention to
Please refer to the details
Following steps
:
1. Washing liquid:
Make
Formulated before use
it is good
, some kits
Instruction manual
Will indicate that
Prepared washing solution, at
4 degrees
Under conditions
Generally can
Save
Put one month
;
in case
Kit instructions
Not marked, try to use fresh
detergent
,Do not
need
More preparation
2. Coloring solution
: coloring liquid is generally divided
Liquid A and liquid B,
Make
Prepared in the first 15 minutes
it is good
;
3. Standards:
most
ELISA
In the kit
of
Standards are already prepared
;still is
4 degrees to save, some are
need
Now prepared, according to
Kit
Instruction manual
get on
operating;
4. Concentrate biotin-conjugated secondary antibody and HRP: if needed, kit
Instruction manual
If you don’t specify how long it can be saved, try to use it now.
Still the same, so
Prepared in the first 15 minutes; principle
on
Is a kit
On the instruction manual
If the storage method of the preparation is not indicated, it should be used now;
This million
Don't be afraid of trouble; there is also a small bottle of tube to be centrifuged before opening the lid, so as not to increase the total amount after the lid is stuck.
use
;
5. Loading: Reagents for incubation at room temperature, especially those with short incubation time, the effect of loading on the data is relatively large, especially pay attention to the problem of loading.
In the majority of scientific research scholars and research projects,
ELISA experiment
Since its inception,
With high sensitivity and good specificity
,
in
each
research
project
on
All
Got
More
Wide range of applications, but
If it is
In operation
Due to
Each link
Not paying attention
The test results have a greater impact on the test.
If these details are
not pay attention to
If you are very
May cause color insufficiency
,or
Flower board and other results.
Shanghai Jiwei Biotechnology Co., Ltd. specializes in the field of ELISA kits for many years. With excellent technology and advanced equipment, the company provides better scientific research reagents for scientific research in China. If you do not understand the place during the operation of ELISA experiments. , you can call our company at any time, consult the relevant technical staff, we will answer you one by one.
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