Principle of identification of immunoglobulin G in pig serum - Master's thesis - Dissertation

Identification principle of immunoglobulin G in pig serum. Porcine immunoglobulin G (IgG) elisa kit provided by Shanghai Jinma Experimental Equipment Co., Ltd.

Pig serum, plasma and related fluid samples

Immunoglobulin G (IgG)

content

. For research use only. This chapter will explain how to detect the content of immunoglobulin G in pig serum. Thank you for your trust and support. If you do not understand, please call us.

Porcine immunoglobulin G (IgG) Elisa kit for the determination of specimens by double antibody sandwich assay

Porcine immunoglobulin G (IgG)

Level. Purified

Porcine immunoglobulin G (IgG)

The antibody is coated with a microplate to prepare a solid phase antibody, which is sequentially added to the microwell of the coated monoclonal antibody.

Immunoglobulin G (IgG),

HRP-labeled

Immunoglobulin G (IgG)

The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color and the sample

Immunoglobulin G (IgG)

Positive correlation. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the sample was calculated from the standard curve.

Porcine immunoglobulin G (IgG)

concentration.
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